Preliminary assessment of small scale bacterial depuration of Crassostrea gigas and Anadara spp., Gulf of Nicoya, Costa Rica

Authors

  • Luis A. Vega-Corrales Universidad Nacional, Costa Rica
  • Carolina Marín-Vindas Universidad Nacional, Costa Rica
  • Oscar Pacheco-Prieto Universidad Nacional, Costa Rica
  • Gerardo Zúñiga-Calero Universidad Nacional, Costa Rica

DOI:

https://doi.org/10.15359/revmar.5.7

Keywords:

Gulf of Nicoya, bacterial depuration, Crassostrea gigas, Anadara spp., E. coli

Abstract

Bivalve molluscs are sold without any sanitary control in Costa Rica, which represents a public health risk due to the possible accumulation of pathogenic bacteria. Small scale bacterial depuration treatments were preliminarily evaluated on Crassostrea gigas and Anadara spp. from the Gulf of Nicoya, Costa Rica, through the use of a recirculating system with UV irradiation. The levels of Escherichia coli in the water were determined using the MPN method. The MPN of E. coli and the presence of Salmonella spp., Vibrio parahaemolyticus and V. cholerae were determined in molluscs. Results confirm the effectiveness of the E. coli depuration system design in C. gigas and Anadara spp. The MPN of E. coli in the molluscs was reduced between 79% and 100% after 24 h. None of the other types of bacteria were found in the samples analyzed. This is the first C. gigas depuration study in Costa Rica and the first report concerning UV depuration for bacterial indicators of fecal contamination in Anadara spp. This research serves as a basis for the implementation and improvement of mollusc purification conditions in the country. It is recommended to monitor extraction and harvesting areas, as well as to implement the depuration of bivalve molluscs so that traditional producers can offer an innocuous product with added value.

Author Biographies

Luis A. Vega-Corrales, Universidad Nacional

Estación de Biología Marina Juan Bertoglia Richards

Carolina Marín-Vindas, Universidad Nacional

Estación de Biología Marina Juan Bertoglia Richards

Oscar Pacheco-Prieto, Universidad Nacional

Estación de Biología Marina Juan Bertoglia Richards

Gerardo Zúñiga-Calero, Universidad Nacional

Estación de Biología Marina Juan Bertoglia Richards

References

Clark, C. (2001). Marine pollution. New York, USA.: Oxford University Press.

Clesceri, L. S., Greenberg, A. E. & Trussell, R. R. (1989). Standard methods for the examination of water and wastewater. Washington, USA.: American Public Health Association, American Water Works Association, Water Pollution Control Federation.

Croci, L., Suffredini, E., Cozzi, L. & Toti, L. (2002). Effects of depuration of molluscs experimentally contaminated with Escherichia coli, Vibrio cholerae 01 and Vibrio parahaemolyticus. J. Appl. Microbiol., 92, 460-465.

Cusson, M., Tremblay, R., Daigle, G. & Roussy, M. (2005). Modeling the depuration potential of blue mussels (Mytilus spp.) in response to thermal shock. Aquaculture, 250, 183- 193.

de Abreu, A., Albarnaz, J. D., Moresco, V., Poli, C. R., Teixeira, A. L., Oliveira, C. M. & Monte, C. R. (2007). Depuration dynamics of oysters (Crassostrea gigas) artificially contaminated by Salmonella enterica serovar Typhimurium. Mar. Environ. Res., 63(5), 479-489.

de Abreu, A., Rigotto, C., Moresco, V., Kleemann, C. R., Teixeira, A. L., Poli, C. R., Oliveira, C. M. & Monte, C. R. (2012). The depuration dynamics of oysters (Crassostrea gigas) artificially contaminated with hepatitis A virus and human adenovirus. Mem. Inst. Oswaldo Cruz, 107(1), 11-17.

Ho, B. S.W. & Tam, T.-Y. (2000). Natural depuration of shellfish for human consumption: a note of caution. Water Res., 34(4), 1401-1406.

Lee, R., Lovatelli, A. & Ababouch, L. (2010). Depuración de bivalvos: aspectos fundamentales y prácticos. FAO Documento Técnico de Pesca No. 511. Rome, Italy: FAO.

López, C., Darriba, S., Miranda, M. & Álvarez, C. (2005). Depuración de la navaja Ensis arcuatus (Jeffreys, 1865) y el longueirón Ensis siliquia (L., 1758) (Solenacea). Bol. Inst. Esp. Oceanogr., 21(1-4), 311-315.

Love, D. C., Lovelace, G. L. & Sobsey, M. D. (2010). Removal of Escherichia coli, Enterococcus fecalis, coliphage MS2, poliovirus, and hepatitis A virus from oysters (Crassostrea virginica) and hard shell clams (Mercinaria mercinaria) by depuration. Int. J. Food Microbiol., 143, 211-217.

MacKenzie, C. L. (2001). The fisheries for mangrove cockles, Anadara spp., from Mexico to Peru, with descriptions of their habitats and biology, the fishermen’s lives, and the effects of shrimp farming. Mar. Fish. Rev., 63(1), 1-39.

Nappier, S. P., Graczyk, T. K. & Schwab, K. J. (2008). Bioaccumulation, retention and depuration of enteric viruses by Crassostrea virginica and Crassostrea ariakensis oysters. Appl. Environ. Microbiol, 74(22), 6825-6831.

Oberbeckmann, S., Fuchs, B. M., Meiners, M., Wichels, A., Wiltshire, K. H. & Gerdts, G. (2012). Seasonal dynamics and modeling of a Vibrio community in coastal waters of the North Sea. Microb. Ecol., 63, 543-551.

Oliveira, J., Cunha, A., Castilho, F., Romalde, J. L. & Pereira, M. J. (2011). Microbial contamination and purification of bivalve shellfish: Crucial aspects in monitoring and future perspectives - A mini-review. Food Control, 22(6), 805-816.

Pouch, F. & Ito, K. (2001). Compendium of methods for the microbiological examination of foods. Washington, USA.: American Public Health Association.

R Core Team. (2012). R: A language and environment for statistical computing. Version 2.15.0. Vienna, Austria: R Foundation for Statistical Computing.

Silva, A. M. & Bonilla, R. (2001). Abundancia y morfometría de Anadara tuberculosa y A. similis (Mollusca: Bivalvia) en el manglar de Purruja, Golfo Dulce, Costa Rica. Rev. Biol. Trop., 49(Supl. 2), 315-320.

Published

2013-12-10

How to Cite

Vega-Corrales, L. A., Marín-Vindas, C., Pacheco-Prieto, O., & Zúñiga-Calero, G. (2013). Preliminary assessment of small scale bacterial depuration of Crassostrea gigas and Anadara spp., Gulf of Nicoya, Costa Rica. Journal of Marine and Coastal Sciences, 5, 107-117. https://doi.org/10.15359/revmar.5.7

Issue

Section

Scientific articles

How to Cite

Vega-Corrales, L. A., Marín-Vindas, C., Pacheco-Prieto, O., & Zúñiga-Calero, G. (2013). Preliminary assessment of small scale bacterial depuration of Crassostrea gigas and Anadara spp., Gulf of Nicoya, Costa Rica. Journal of Marine and Coastal Sciences, 5, 107-117. https://doi.org/10.15359/revmar.5.7

Comentarios (ver términos de uso)