Identification of four species of Clupeiformes (Actinopterygii) through analysis of mitochondrial DNA sequences in fishing exploitation areas of the Gulf of Nicoya, Costa Rica

Keywords: Opisthonema spp., Cetengraulis sp, mtDNA, rDNA, taxonomic placement


Sardines, together with anchovies, constitute one of the most important groups in the fishery of the Gulf of Nicoya. They are consumed by large fish and seabirds; therefore, they not only suffer from fishing exploitation but also have high rates of natural mortality. Due to their commercial and ecological importance, it is necessary to have a reliable taxonomic identification of these species, mainly for the preparation of fisheries studies. Previously, the identification of fish species was based only on external morphological characteristics; however, this is not always possible due to their morphological similarity, but the combined use of morphological and genetic data based on mitochondrial genes can contribute to their accurate recognition. Historically, taxonomic studies have grouped the species O. libertate, O. medirastre and O. bulleri as members of the Opisthonema spp. complex due to their great morphological similarity, while, there are few taxonomic studies  of the family Engraulidae. In the present work, species of the Opisthonema spp. complex and a species of the genus Cetengraulis sp., collected between June-December 2017, were molecularly identified by sequencing mitochondrial genes: COI, Ctyb, and 16S rRNA. The findings of the molecular data analysis confirm the identity and presence of three species of the Opisthonema spp. complex and a species of anchovy, in areas of fishing exploitation in the gulf of Nicoya, Costa Rica, information that facilitates the use and management of these fishing resources in a marine area of ​​commercial importance for the country.


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Author Biographies

Carolina Sancho-Blanco, Universidad Nacional

Escuela de Ciencias Biológicas, Laboratorio de Análisis Genómico (LAGEN).

Luis Hernández-Noguera, Universidad Nacional

Estación de Biología Marina Juan Bertoglia Richards, Escuela de Ciencias Biológicas, Laboratorio de Análisis Biológicos-Pesqueros

Luis Vega-Alpízar, Universidad Nacional

Estación de Biología Marina Juan Bertoglia Richards, Escuela de Ciencias Biológicas, Laboratorio de Análisis Biológicos-Pesqueros

Rosa Soto-Rojas, Universidad Nacional

Estación de Biología Marina Juan Bertoglia Richards, Escuela de Ciencias Biológicas, Laboratorio de Análisis Biológicos-Pesqueros

Rodolfo Umaña-Castro, Universidad Nacional

Escuela de Ciencias Biológicas, Laboratorio de Análisis Genómico (LAGEN).


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How to Cite
Sancho-Blanco, C., Hernández-Noguera, L., Vega-Alpízar, L., Soto-Rojas, R., & Umaña-Castro, R. (2021). Identification of four species of Clupeiformes (Actinopterygii) through analysis of mitochondrial DNA sequences in fishing exploitation areas of the Gulf of Nicoya, Costa Rica. Journal of Marine and Coastal Sciences, 13(1), 39-56.
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