Comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (New Jersey and Indiana serotypes) in equines from Costa Rica

Authors

  • Maren von Koeckritz-Blickwede Institut fuer Physiologische Chemie, Tieraerztliche Hochschule Hannover, Buenteweg 17, 30559 Hannover, Germany, Germany
  • Gaby Dolz Wiedner Universidad Nacional Escuela de Medicina Veterinaria Laboratorio de Entomología, Costa Rica
  • Marco Vinicio Herrero Universidad Nacional Escuela de Medicina Veterinaria Laboratorio de Entomología, Costa Rica

Keywords:

vesicular stomatitis virus, serological diagnosis, neutralizing antibodies, competitive ELISA, horses, Costa Rica

Abstract

A competitive enzyme-linked immunosorbent assay (c-ELISA) was compared to the serum neutralization test (SNT) for the detection of antibodies against vesicular stomatitis virus, New Jersey (VSV-NJ) and Indiana (VSV-IN) serotypes, using 214 equine serum samples from Costa Rica. In the c-ELISA for VSV-NJ 109 (50.93%) equine sera reacted positive, whereas in the SNT 138 (64.48%) serum samples reacted positive to VSV-NJ. In the c-ELISA for VSV-IN 26 (12.15%) sera reacted positive, whereas in the SNT 36 (16.83%) equine sera reacted positive to VSV-IN. The c-ELISA showed a moderate sensitivity compared to the SNT. The rapidity and ease of the c-ELISA system makes it suitable as a rapid diagnostic screening assay. However, because of the lower sensitivity of the c-ELISA, the SNT is considered the method of choice to detect equine VSV infections in Costa Rica.

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Published

2015-09-12

How to Cite

Comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (New Jersey and Indiana serotypes) in equines from Costa Rica. (2015). Ciencias Veterinarias, 32(1), 21-27. https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/6963

How to Cite

Comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (New Jersey and Indiana serotypes) in equines from Costa Rica. (2015). Ciencias Veterinarias, 32(1), 21-27. https://www.revistas.una.ac.cr/index.php/veterinaria/article/view/6963

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