Antioxidant defenses in the coelomic fluid of Echinometra lucunter (Linnaeus, 1758) stimulated with bacterial inoculums

  • Edgar Zapata-Vívenes Departamento de Biología, Escuela de Ciencias, Núcleo de Sucre, Universidad de Oriente, Cumaná, Venezuela. Escuela de Acuicultura y Pesca, Facultad de Ciencias Veterinarias, Universidad Técnica de Manabí, Bahía de Caráquez, Manabí, Ecuador
  • Gabriela Aparicio Departamento de Bioanálisis, Escuela de Ciencias, Núcleo de Sucre, Universidad de Oriente, Cumaná, Venezuela.
Keywords: Catalase, lipid peroxidation, oxidative stress, E. coli, thiols


Phagocytosis is a first-line cellular response mediated by specialized cells denominated coelomocytes- amoebocytes. This process allows for the inclusion of extraneous particles or microorganisms, which are eliminated by the production of reactive oxygen species (ROS). In order to evaluate the antioxidant defense system in the coelomic fluid (CF) of E. lucunter under elevated phagocytic activity (PA), three bacterial strains were inoculated separately, via peristomial membrane: E. coli, V. parahaemolyticus and M. lysodeikticus. The phagocytic capacity (PC), catalase (CAT) and superoxide dismutase (SOD) activities, levels of lipoperoxidation (LPO), sulphydryl groups (-SH) and proteins were determined at 16 h post-injection. In addition, the time of righting response of each individual was noted. The PA, CAT, and proteins showed increments in the organisms inoculated with bacteria. The LPO, SOD, and -SH levels showed no variations among the experimental organisms. The time of righting response showed slight variations in the bacteria-stimulated organisms, as well as a low percentage of lost and reduced movements of their spines and tube feet. The PA and proteins in CF of E. lucunter show the effectiveness of the immune system in the presence of microbial stimulants. Results indicate that CAT plays a preponderant role in the CF to avoid changes in the antioxidant status, associated with the respiratory burst during elevated phagocytic activities. Antioxidant responses of E. lucunter immunostimulated by bacteria may guarantee their survival in their natural habitat. 


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Aebi, H. (1984). Catalase in vitro. Meth. Enzimol., 105, 121-126.
Anton, Y. & Salazar, R. (2009). El sistema inmune de los invertebrados. Revista Electrónica de Veterinaria, 10, 1-10.
Baba, S. P. & Bhatnagar, A. (2018). Role of thiols in oxidative stress. Curr. Opin. Toxicol.
Bastidas, M. (2017). Respuestas inmunológicas innatas en el erizo verdi-blanco Lytechinus variegatus (Echinoidea: Toxopneustidae) infectado experimentalmente con bacterias. Trabajo de grado no publicado. Universidad de Oriente, Venezuela.
Bauer, J. C. & Young, C. M. (2000). Epidermal lesions and mortality caused by vibriosis in deep-sea Bahamian echinoids: a laboratory study. Dis. Aquat. Org., 39, 193-199. Doi: 10.3354/dao039193.
Beaumont, T. (2010). The free radical theory of ageing: does it apply to Antarctic and temperate sea urchin. Tesis de doctorado no publicada. University of Atago, New Zealand.
Branco, P., Figueiredo D. & Da Silva J. (2014). Nuevos conocimientos sobre el sistema inmunitario innato de erizo de mar: coelomocytes como biosensores para el estrés ambiental. OA Biología, 2, 2.
Buggé, D. M., Hégaret, H., Wikfors, G. H. & Allam, B . (2006). Oxidative burst in hard clam (Mercenaria mercenaria) haemocytes. Fish Shell. Immunol., 23, 188-196.
Campa-Cordova, A., Hernández-Saavedra, N. Y. & De Pilippis, R. (2002). Generation of superoxide anion and SOD activity in hemocytes and muscle of American white shrimp (Litopenaeus vannamei) as a response to β-glucan and sulphate polysaccharide. Fish Shell. Inmunol., 12, 353-366.
Chihuailaf, R., Contreras, P. & Wittwer, F. (2002). Patogénesis del estrés oxidativo: Consecuencias y evaluación en salud animal. Veterinaria México, 33, 265-283.
Cipriano-Maack, A. N. (2016). Immunostimulatory effects of different aspects of aquaculture on the host response in the edible sea urchin, Paracentrotus lividus. Tesis de doctorado no publicada, University College Cork, Ireland.
Cooper, E., Raftos, D., Zhang, Z. & Kelly, K. (1995). Purification and characterisation of tunicate opsonins and cytokine-like proteins. In J. S. Stolen (Ed). Techniques in Fish Immunology-4: Immunology and pathology of aquatic invertebrates (pp. 43-54). Fair Haven, EE. UU.: SOS Publications.
Couter, G., Warnau, M., Jangoux, M. & Dubois, P. (1999). Reactive oxygen species (ROS) production by amoebocytes of Asterias rubens (Echinodermata). Fish. Shell. Immunol., 12, 187-200.
Dale, B. & Russo, P. (1988). Sulfhydryl groups are involved in the activation of sea urchin eggs. Gam. Res., 19(2), 161-168.
Dheilly, N. (2010). Proteomic analysis of sea urchin immune responses and characterization of highly variable immune response proteins. Tesis de doctorado no publicada. Sydney, NSW, Australia.
Dheilly, N., Haynes, P., Raftos, D. & Nair S. (2012). Time course proteomic profiling of cellular responses to immunological challenge in the sea urchin, Heliocidaris erythrogramma. Develop. Comp. Immunol., 37, 243-256.
Donaghy, L., Kraffe, E., Le Goıc, N., Lambert, C., Volety, A. K. & Soudant, P. (2012). Reactive oxygen species in unstimulated hemocytes of the pacific oyster Crassostrea gigas: A mitochondrial involvement. PLoS ONE, 7, e46594.
Dolmatova, L., Elisekina, M., Timchenko, N., kovalera, A., & Shitkova, O. (2003). Generation of reractive oxygen species in the different fractions of the coclomocytes of holothurian Eupentacia fraundatris in response to the thermostable toxin of Yersenia pseudotuberculosis in vitro. Chinese J Lim. Oceanol., 21(4), 293-304.
Ellman, G. L. (1959). Tissue sulfhydryl groups. Arch. Biochem. Biophys., 82, 70-77.
Halliwell, B. & Gutteridge, J. M. C. (2015). Free Radicals in Biology and Medicine. New York, EE. UU.: Oxford University Press.
Holmblad, T. & Söderhäll, K. (1999). Cell adhesion molecules and antioxidant enzymes in a crustacean, possible role in immunity. Aquaculture, 172, 111-123.
Lawrence, J. & Balzhin, A. 1998. Life-history strategies and the potential of sea urchins for aquaculture. J Shellfish. Res., 17, 1515-1522.
Majeske, A. J., Bayne, C. J. & Smith, L. C. (2013). Aggregation of sea urchin phagocytes is augmented in vitro by lipopolysaccharide. Publ. Libr. Sci., 8, 61419.
Matranga, V., Pinsino, A., Celi, M., Natoli, A., Bonaventura, R., Schröder, H. C. &, Müller, W. E. G. (2005) Monitoring chemical and physical stress using sea urchin immune cells. In V. Matranga (Ed) Echinodermata (pp. 85-110), New York, EE. UU.: Springer.
Matranga, V., Pinsino, A., Celi, M., Di Bella, G. & Natoli, A. (2006). Impacts of UV-B radiation on short-term cultures of sea urchin coelomocytes. Mar. Biol., 149, 25-34.
Mydlarz, L., Jones, L. & Drew Harvell, C. (2006). Innate Immunity, environmental drivers, and disease ecology of marine and freshwater invertebrates. An. Rev. Ecol. Evol. System., 37, 251-88.
Nebot, C., Moutet, M., Huet, P., J. Yadan & Chau-diere, J. (1993). Spectrophotometric assay of superoxide dismutase activity based on the activated autoxidation of a tetracyclic catechol. Anal. Biochem., 214, 442-451.
Ohkawa, H., Ohishi N. & Yagi, K. (1978). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Rev. Anal. Biochem., 95(2), 351-358.
Ovando, P. (2009). Caracterización celular y molecular de la respuesta inmune en el erizo antártico Sterechinus neumayeri. Tesis de grado no publicada. Universidad de Magallanes, Chile.
Pinsino, A. & Matranga, V. (2015). Sea urchin immune cells as sentinels of environmental stress. Dev. Comp. Immunol., 49, 198-205.
Pipe, R. (1992). Generation of reactive oxygen metabolites by the hemocytes of the mussel Mytilus edulis. Dev. Comp. Immunol., 17(1-2), 211-219.
Reyes-Luján, J., Arrieche, D., Lodeiros-Seijo, C., Zapata-Vívenes, E., Barrios, J. & Salgado, W. (2015). Ciclo gametogénico de Echinometra lucunter (Linnaeus 1758) (Echinometra: Echinoidea) en el Nororiente de Venezuela. Rev. Biol. Trop., 63, 273-283.
Robinson, H. & Hogden, C. (1940). Relationship to the protein which bears a quantitative production of a stable color conditions necessary for the proteins: a study of the determination of serum concentration. J. Biol. Chem., 35, 707-725.
Roch, P. (1999). Defense mechanisms and disease prevention in farmed marine invertebrates. Aquaculture, 172 (1-2), 125-145.
Sokal, R. & Rohlf, J. (1981). Biometry: The Principles and Practice of Statistics in Biological Reasearch. New York, EE. UU.: WH Freeman.
Storey, K. (1996). Oxidative stress: animal adaptations in nature. Braz. J. Med. Biol. Res., 29(12), 1715-1733.
Taylor, J., Lovera, C., Whaling, P., Buck, K., Pane, E. & Barry, J. (2014). Physiological effects of environmental acidification in the deep-sea urchin Strongylocentrotus fragilis. Biogeosciences, 11, 1413-1423.
Tucunduva-Faria, M. & Machado-Cunha da Silva, J. (2008). Innate immune response in the sea urchin Echinometra lucunter (Echinodermata). J. Invert. Pathol., 98, 58-62.
Van-Laer, K., Hamilton, C. & Messens, J. (2013). Low-Molecular-Weight Thiols in Thiol-Disulfide Exchange. Antiox. Red. Signal., 18(13), 1642-1653.
Wang, Y., Feng, N., Li, Q., Ding, J., Zhan, Y. Y. & Chang, Y. Q. (2012a). Isolation and characterization of bacteria associated with a syndrome disease of sea urchin Strongylocentrotus intermedius in North China. Aquacult. Res., 44, 1-10.
Wang, Y., Chang, Y. Q. & Lawrence J. M. (2012b). Disease in sea urchin. In J. Lawrence, (Ed), Edible Sea Urchins: Biology and Ecology (pp. 179-186). Amsterdam, Netherlands: Elsevier
Yeh, S. T. & Chen, J. C. (2008). Immunomodulation by carrageenans in the white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus. Aquaculture, 276(1-4), 22-28.
How to Cite
Zapata-Vívenes, E., & Aparicio, G. (2019). Antioxidant defenses in the coelomic fluid of Echinometra lucunter (Linnaeus, 1758) stimulated with bacterial inoculums. Journal of Marine and Coastal Sciences, 11(1), 27-42.

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