Review article: Fish sperm quality and assessment methods

Authors

  • Juan C. López-Hernández Universidad Juárez Autónoma de Tabasco, Mexico
  • Adriana Osorio-Pérez Universidad Juárez Autónoma de Tabasco, Mexico
  • Salvador A. Jiménez-Félix Universidad Juárez Autónoma de Tabasco, Mexico
  • Salomón Páramo-Delgadillo Universidad Juárez Autónoma de Tabasco, Mexico
  • Gabriel Márquez-Couturier Universidad Juárez Autónoma de Tabasco, Mexico
  • George S. Yasui Universidade de São Paulo, São Paulo, Brasil., Brazil
  • Lenin Arias-Rodríguez Universidad Juárez Autónoma de Tabasco, Mexico

DOI:

https://doi.org/10.15359/revmar10-1.5

Keywords:

Sperm quality, assessment, methods, fish, reproduction

Abstract

Aspects such as anthropic effects, loss of biodiversity, species displacement, introduction of exotic species, and demographic growth have caused an increase in crop yield, recovery of impacted sites, reintroduction of species and the search for alternatives to understand and evaluate reproduction of native fish species. In such context, knowing and using both female and male gametes for massive larvae production creates the need to use methodologies that would allow sorting the brood stock with genetic and physiological traits that would improve the quantity and quality of larvae to be used for massive culture, restocking, breeding production, and experiments on basic and applied sciences. This bibliographic review mentions the particularities of fish sperm cells and methodological alternatives to assess quality, by understanding activation physiology (motility percentages and times, type of movement and sperm number), including the structural analysis at extracellular and intracellular levels (spermatic morphology, damage to the membrane, comet assay, live/dead/apoptotic cells, which depend on the main objectives of reproduction or experimental programs). The development of automated cytometry and unicellular genomics equipment has allowed for a better understanding of the functioning of spermatic cells, genetic expression linked to cellular qualities and their biological implication.

Author Biographies

Juan C. López-Hernández, Universidad Juárez Autónoma de Tabasco

División Académica de Ciencias Biológicas

Adriana Osorio-Pérez, Universidad Juárez Autónoma de Tabasco

División Académica de Ciencias Biológicas

Salvador A. Jiménez-Félix, Universidad Juárez Autónoma de Tabasco

División Académica de Ciencias Biológicas

Salomón Páramo-Delgadillo, Universidad Juárez Autónoma de Tabasco

División Académica de Ciencias Biológicas

Gabriel Márquez-Couturier, Universidad Juárez Autónoma de Tabasco

División Académica de Ciencias Biológicas

George S. Yasui, Universidade de São Paulo, São Paulo, Brasil.

Universidade de São Paulo, São Paulo, Brasil.

Lenin Arias-Rodríguez, Universidad Juárez Autónoma de Tabasco

División Académica de Ciencias Biológicas

References

Aas, G. H., Refstie, T. & Gjerde, B. (1991). Evaluation of milt quality of Atlantic salmon. Aquaculture, 95, 125-132. https://doi.org/10.1016/0044-8486(91)90079-M.

Aksoy, E., Aktan, T. M., Duman, S. & Cuce, G. (2012). Assessment of spermatozoa morphology under light microscopy with different histology stains and comparison of morphometric measurements. Int. J. Morphol, 30(4), 1544-1550. https://doi.org/10.4067/S0717-95022012000400045.

Alavi, S. M. H., Rodina, M., Viveiros, A. T. M., Cosson, J., Gela, D., Boryshpolets, S. & Linhart, O. (2009). Effects of osmolality on sperm morphology, motility and flagelar wave parameters in Northern pike (Esox lucius L.). Theríogenology, 72, 32-43. https://doi.org/10.1016/j.theriogenology.2009.01.015.

Arias-Rodríguez, L. (2001). Inactivación genética de esperma e inducción de ginogénesis y de triploidía en el botete diana Sphoeroides annulatus, (Jenyns, 1842). Tesis de maestría no publicada, Centro de Investigación para la Alimentación y Desarrollo, A. C. (CIAD), Unidad Mazatlán, Sinaloa, México.

Arias-Rodríguez, L., Rodríguez, I. L. E. & Pignataro, V. G. (2004). Efecto de la radiación UV en la inactivación genética del esperma de botete diana Sphoeroides annulatus (Jenyns, 1842). Ciencias Marinas, 30, 390-402. https://doi.org/10.7773/cm.v30i3.334.

Arias-Rodríguez, L., Yasui, G. J., Kusuda, S., Fujimoto, K. & Arai, K. (2010). Reproductive and genetic capacity of spermatozoa of inter-populational hybrid males in the loach, Misgurnus anguillicaudatus. J. Appl. Ichthyol, 26, 653-658. https://doi.org/10.1111/j.1439-0426.2010.01534.x.

Álvarez, B., Arenal, A., Fuentes, R., Pimentel, R., Abad, Z. & Pimentel, E. (2009). Methods in reproductive aquaculture: Marine and Freshwater Species. In E. Cabrita., V. Robles & P. Herraez (Eds.), Use of post-thaw silver carp (Hypophtalmichthys molitrix) spermatozoa to increase hatchery productions (pp. 345-350). Florida, EE. UU.: C. R. C. Press.

Billard, R. (1986). Spermatogenesis and spermatology of some teleost fish species. Reprod. Nutr. Dévelop., 26(4), 877-920. https://doi.org/10.1051/rnd:19860601.

Billard, R., Cosson, J., Crim, L. W. & Suquet, M. (1995a). Broodstock managament and egg and larval quality. In N. R. Bromage & R. J. Robert (Eds.), Sperm physiology and quality (pp. 25-53). Boston, EE. UU.: Blackwell Science, Oxford.

Billard, R., Cosson, J., Perchec, G. & Linhart, O. (1995b). Biology of sperm and artificial reproduction in carp. Aquaculture, 129, 95-112. https://doi.org/10.1016/0044-8486(94)00231-C.

Birkhead, T. R., Hosken, D. J. & Pitnick, S. (2009). Sperm biology: An evolutionary perspective. Burlington, EE. UU.: ELSEVIER.

Bobe, J. & Labbé, C. (2010). Egg and sperm quality in fish. General and Comparative Endocrinology, 165(3), 535-548. https://doi.org/10.1016/j.ygcen.2009.02.011.

Brana, C., Benham, C. & Sundstrom, L. (2002). A method for characterizing cell death “in vitro” by combining propidium iodide staining with immunohistochemistry. Brain Res. Protocols, 10(2), 109-114. https://doi.org/10.1016/S1385-299X(02)00201-5.

Cabrita, E., Martínez-Páramo, S., Gavaia, P. J., Riesco, M. F., Valcarce, D. G., Sarasquete, C., Herráez, M. P. & Robles, V. (2014). Factors enhancing fish sperm quality and emerging tools for sperm analysis. Aquaculture. 432, 389-401. https://doi.org/10.1016/j.aquaculture.2014.04.034.

Chalah, T. & Brillard, J. P. (1998). Comparison of assessment of fowl sperm viability by eosin-nigrosin and dual fluorescence (SYBR-14/PI). Theriogenology, 50(3), 487-493. https://doi.org/10.1016/S0093-691X(98)00155-1.

Chan, L. L., Wilkinson, A. R., Paradis, B. D. & Lai, N. (2012). Rapid image-based cytometry for comparison of fluorescent viability staining methods. J. Fluores., 22(5), 1301-1311. https://doi.org/10.1007/s10895-012-1072-y.

Chapman, G. V. (2000). Instrumentation for flow cytometry. J. Immunol. Methods, 243(1), 3-12. https://doi.org/10.1016/S0022-1759(00)00224-6.

Ciereszko, A., Dietrich, M. A. & Nynca, J. (2017). Fish semen proteomics - New opportunities in fish reproductive research. Aquaculture, 472, 81-92. https://doi.org/10.1016/j.aquaculture.2016.03.005.

Ciereszko, A., Wolfe, T. D. & Dabrowskia, K. (2005). Analysis of DNA damage in sea lamprey (Petromyzon marinus) spermatozoa by UV, hydrogen peroxide, and the toxicant bisazir. Aquat. Toxicol., 73, 128-138. https://doi.org/10.1016/j.aquatox.2005.03.003.

Cosson, J., Groison, A. L., Suquet, M. & Fauvel, C. (2008). Motility characteristics of spermatozoa in cod (Gadus morhua) and hake (Merluccius merluccius). Cybium, 32, 176-177.

Costache, M., Radu, D., Nicolae, C. G. & Grosu, H. (2012). Relationships between morphology, motility and fertilization capacity in paddlefish (Polyodon spathula) spermatozoa. Adv. Res. Sci. Areas, 24(4), 1427-1429. https://doi.org/10.1111/j.1439-0426.2008.01145.x.

Coward, K., Bromage, N. R., Hibbitt, O. & Parrington, J. (2002). Gamete physiology, fertilization and egg activation in teleost fish. Rev. Fish Biol. Fish., 12, 33-58. https://doi.org/10.1023/A:1022613404123.

Dreanno, C., Suquet, M., Desbruyeres, E., Cosson, J., Delliou, H. L. & Billard, R. (1998). Effect of urine on semen quality in turbot (Psetta maxima). Aquaculture, 169, 247-262. https://doi.org/10.1016/S0044-8486(98)00262-2.

Duan, W., Xu, K., Hu, F., Zhang, Y., Wen, M., Wang, J., Tao, M., Luo, K., Zhao, R., Qin, Q., Zhang, C., Liu, J., Liu, Y. & Liu, S. (2016). Comparative Proteomic, Physiological, Morphological, and Biochemical Analyses Reveal the Characteristics of the Diploid Spermatozoa of Allotetraploid Hybrids of Red Crucian Carp (Carassius auratus) and Common Carp (Cyprinus carpio). Biology of Reproduction, 94, (1-2)35, 1-12. https://doi.org/10.1095/biolreprod.115.132787.

Dumorné, K., Figueroa, E., Cosson, J., Lee-Estevez, M., Ulloa-Rodríguez, P., Valdebenito, I. & Farías, J. C. (2017). Protein phosphorylation and ions effects on salmonid sperm motility activation, Rev. Aquaculture, 0, 1-11. https://doi.org/10.1111/raq.12198.

Dziewulska, K., Rzemieniecki, A., Baranowska-Bosiacka, I., Domagała, J. & Chlubek, D. (2012). The parameters of energetic status of the wild Atlantic salmon (Salmo salar L.) spermatozoa. Aquaculture Res., 43(1), 139-148. https://doi.org/10.1111/j.1365-2109.2011.02833.x.

Dzyuba, B., Bondarenko, O., Fedorov, P., Gazo, I., Prokopchuk, G. & Cosson, J. (2017). Energetics of fish spermatozoa: The proven and the possible. Aquaculture, 472, 60-72. https://doi.org/10.1016/j.aquaculture.2016.05.038.

Dzyuba, V. & Cosson, J. (2014). Motility of fish spermatozoa: from external signaling to flagella response. Reprod. Biol., 14 (3), 165-175. https://doi.org/10.1016/j.repbio.2013.12.005.

Esteso, M. C., Rodríguez, E., Toledano-Díaz, A., Castaño, C., Pradiee, J., López-Sebastián, A. & Santiago-Moreno, J. (2015). Descriptive analysis of sperm head morphometry in Iberian ibex (Capra pyrenaica): Optimum sampling procedure and staining methods using Sperm-Class Analyzer®. Anim. Reprod. Sci., 155, 42-49. https://doi.org/10.1016/j.anireprosci.2015.01.014.

Fauvel, C., Suquet, M. & Cosson, J. (2010). Evaluation of fish sperm quality. J. Appl. Ichthyol., 26(5), 636-643. https://doi.org/10.1111/j.1439-0426.2010.01529.x.

Fribourgh, J. H. (1966). The application of a differential staining method to low-temperature studies on goldfish spermatozoa. The Prog. Fish-Culturist, 28(4), 227-231. https://doi.org/10.1577/1548-8640(1966)28[227:TAOADS]2.0.CO;2.

Glogowski, J., M. Kwasnik., B. Piros., K. Dabrowski., K. Goryczko., S. Dobosz., H. Kuzminski & A. Cierezko. (2000). Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success. Aquaculture Res., 31, 289-296. https://doi.org/10.1046/j.1365-2109.2000.00400.x.

Graham, J. K. (2001). Assessment of sperm quality: a flow cytometric approach. Anim. Reprod. Sci., 68, 239-247. https://doi.org/10.1016/S0378-4320(01)00160-9.

Herráez, M. P., Ausió, J., Devaux, A., González-Rojo, S., Fernández-Díez, C., Bony, S., Saperas, N. & Robles, V. (2017). Paternal contribution to development: Sperm genetic damage and repair in fish. Aquaculture, 472, 45-59. https://doi.org/10.1016/j.aquaculture.2016.03.007.

Islam, M. S. & Akhter, T. (2011). Tale of fish sperm and factors affecting sperm motility: A Review. Adv. Life Sci., 1, 11-19. https://doi.org/10.5923/j.als.20110101.03.

Jamieson, B. G. M. (1991). Fish evolution and systematics: evidence from spermatozoa. With a survey of lophophorate, echinoderm and protochordate sperm and an account of gamete cryopreservation. New York, USA: Cambridge University Press.

Jiménez-Trinidad, A. (2016). La osmolaridad y sus efectos en la vitalidad espermática del bagre tropical Rhamdia laticauda (Pisces: Heptapteridae). Tesis de Licenciatura no publicada, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, México.

Koopman, G., Reutelingsperger, C. P. M., Kuijten, G. A. M., Keehnen, R. M. J., Pals, S. T. & Vans Oers, M. H. J. (1994). Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood, 84(5), 1415-1420.

Lahnsteiner, F. & Patzner, R. A. (1998). Sperm motility of the marine teleosts Boops boops, Diplodus sargus, Mullus barbatus and Trachurus mediterraneus. J. Fish Biol., 52, 726-742. https://doi.org/10.1111/j.1095-8649.1998.tb00816.x.

Lahnsteiner, F., Berger, B., Weismann, T. & Patzner, R. A. (1998). Determination of semen quality of the rainbow trout, Oncorhynchus mykiss, by sperm motility, seminal plasma parameters, and spermatozoa metabolism. Aquaculture, 163, 163-181. https://doi.org/10.1016/S0044-8486(98)00243-9.

Linhart, O., Cosson, J., Mims, S., Rodina M., Gela, D. & Shelton, W. L. (2003). Effects of ions on the motility of fresh and demembranate spermatozoa of common carp (Cyprinus carpio) and paddlefish (Polyodon spathula). Fish Physiol. Biochem., 28(1-4), 203-5. https://doi.org/10.1023/B:FISH.0000030528.03098.6d.

López-Hernández, J. C. (2013). Efecto de la luz ultravioleta sobre la calidad espermática del juil tropical Rhamdia laticauda (Pisces: Heptapteridae). Tesis de Licenciatura no publicada, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, México.

López-Hernández, J. C. (2017). Biología, fisiología espermática básica y efecto de la radiación ultravioleta sobre la fisiología e integridad de las células espermáticas del bagre tropical Rhamdia laticauda. Tesis de Maestría no publicada, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, México.

Mattei, X., Siau, Y., Thiaw, O. T. & Thain, D. (1993). Peculiarities in the organization of testis Ophidion sp. (Pisces Teleosts). Evidence for two types of spermatogenesis in teleost fish. J. Fish. Biol., 43(6), 931-937. https://doi.org/10.1111/j.1095-8649.1993.tb01167.x.

Menkveld, R. & Kruger, T. E. (1996). Human Spermatozoa in Assisted Reproduction. In A. A. Acosta & T. F. Kruger (Eds.), Basic semen analysis (pp. 53-72). New York, EE. UU.: Parthenon Publishing Group.

Miliorini, A. B., Murgas, L. D. S., Rosa, P. V., Oberlender, G., Pereira, G. J. M. & da Costa, D. V. (2011). A morphological classification proposal for curimba (Prochilodus lineatus) sperm damages after cryopreservation. Aquaculture Res., 42(2), 177-187. https://doi.org/10.1111/j.1365-2109.2010.02575.x.

Murakami, E., Rodrigueiro, R. J. B., Santos, T. C., Ospina-Rojas, I. C. & Rademacher, M. (2014). Effects of dietary supplementation of meat-type quail breeders with guanidinoacetic acid on their reproductive parameters and progeny performance. Poult. Sci., 93(9), 2237-2244. https://doi.org/10.3382/ps.2014-03894.

Mylonas, C. C., Duncan, N. J. & Asturiano, J. F. (2017). Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality. Aquaculture, 472, 21-44. https://doi.org/10.1016/j.aquaculture.2016.04.021.

Mylonas, C. C., Fostier, A. & Zanuy, S. (2010). Broodstock management and hormonal manipulations of reproduction. Gen. Comp. Endoc., 165(3), 516-534. https://doi.org/10.1016/j.ygcen.2009.03.007.

Osorio-Pérez, A. (2017). Fisiología y microestructura espermática del pejelagarto tropical Atractosteus tropicus. Tesis de Licenciatura no publicada, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, México.

Patzner, R. A. (2008). Reproductive strategies of fish. In M. R. Rocha., A. Arukwe & B. G. Kapoor (Eds.), Fish reproduction (pp. 311-351). New Hampshire, EE. UU.: Science Publishers.

Perchec, G., Jeulin, C., Cosson, J., André, F. & Billard, R. (1995). Relationship between sperm ATP content and motility of carp spermatozoa. J. Cell Sci., 108, 747-753.

Psenicka, M., Alavi, S. M. H., Rodina, M., Cicova, Z., Gela, D., Cosson, J., Nebesarova, J. & Linhart, O. (2008). Morphology, chemical contents and physiology of chondrostean fish sperm: a comparative study between Siberian sturgeon (Acipenser baerii) and sterlet (Acipenser ruthenus). J. Appl. Ichthyol, 24(4), 371-377. https://doi.org/10.1111/j.1439-0426.2008.01139.x.

Psenicka, M., Alavi, S. M. H., Rodina, M., Gela, D., Nebesarova, J. & Linhart, O. (2007). Morphology and ultrastructure of Siberian sturgeon (Acipenser baerii) spermatozoa using scanning and transmission electron microscopy. Biol. Cell., 99(2), 103-115. https://doi.org/10.1042/BC20060060.

Quagio-Grassiotto, I. & Oliveira, C. (2008). Sperm ultrastructure and a new type of spermiogenesis in two species of Pimelodidae, with a comparative review of sperm ultrastructure in Silurifomes (Teleostei: Ostaríophysis). Zool. Anz., 247(1), 55-66. https://doi.org/10.1016/j.jcz.2007.07.002.

Rakitin, A., Ferguson, M. M. & Trippel, E. A. (1999). Spermatocrit and spermatozoa density in Atlantic cod (Godus morhua): correlation and variation during the spawning season. Aquaculture, 170(3-4), 343-358. https://doi.org/10.1016/S0044-8486(98)00417-7.

Rodríguez-Gutiérrez, M. (1992). Técnicas de evaluación cuantitativa de la madurez gonádica de peces. México: A. G. T. Editor.

Rurangwa, E., Kime, D. E., Ollevier, F. & Nash, J. P. (2004). The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture, 234(1-4), 1-28. https://doi.org/10.1016/j.aquaculture.2003.12.006.

Satterfield, J. R. & Flickinger, S. A. (1995). Factors influencing storage potencial of preserved walleye semen. Prog. Fish Cult., 57(3), 175-181. https://doi.org/10.1577/1548-8640(1995)057<0175:FISPOP>2.3.CO;2.

Saudrais, C., Fierville, F., Loir, M., Le Rumeur, E., Cibert, C. & Cosson, J. (1998), The use of phosphocreatine plus ADP as energy source for motility of membrane-deprived trout spermatozoa. Cell Motil. Cytoskel., 41(2), 91-106. https://doi.org/10.1002/(SICI)1097-0169(1998)41:2<91::AID-CM1>3.0.CO;2-I.

Schreck, C. B. & Moyle, P. B. (1990). Methods for Fish Biology. Maryland, EE. UU.: American Fisheries Society.

Schulz, R. W., França, L. R., Lareyre, J. J., LeGac, F., Chiarini-Garcia, H., Nobrega, R. H. & Miura, T. (2010). Spermatogenesis in fish. Gen. Comp. Endocr., 165(3), 390-411. https://doi.org/10.1016/j.ygcen.2009.02.013.

Schutte, B., Nuydens, R., Geerts, H. & Ramaekers, F. (1998). Annexin V binding assay as a tool to measure apoptosis in differentiated neuronal cells. J. Neurosci. Methods, 86(1), 63-69. https://doi.org/10.1016/S0165-0270(98)00147-2.

Serafini, R., Longobardi, V., Spadetta, M., Neri, D., Aríota, B., Gasparrini, B. & Di Palo, R. (2014). Trypan blue/giemsa staining to assess sperm membrane integrity in Salernitano stallions and its relationship to pregnancy rates. Reprod. Dom. Anim., 49(1), 41-47. https://doi.org/10.1111/rda.12221.

Taati, M. M., Mehrad, B., Shabani, A. & Golpour, A. (2010). Correlation between chemical composition of seminal plasma and sperm motility characteristics of Prussian carp (Carassius gibelio). Int. J. Bioflux Soc., 3(3), 233-238.

Tabares, C. J., Tarazona, A. M. & Olivera-Ángel, M. (2005). Fisiología de la activación del espermatozoide en peces de agua dulce. Rev. Colomb. Cienc. Pec., 18(2), 149-161.

van der Horst, G. & Maree, L. (2010). Sperm Blue®: a new universal stain for human and animal sperm which is also amenable to automated sperm morphology analysis. Biotech. Histochem., 84(6), 299-308. http://dx.doi.org/10.3109/10520290902984274.

Vermes, I., Haanen, C. & Reutelingsperger, C. (2000). Flow cytometry of apoptotic cell death. J. Immunol. Methods, 243(1-2), 167-190. https://doi.org/10.1016/S0022-1759(00)00233-7.

Wootton, R. J. & Carl, S. (2014). Reproductive Biology of Teleost Fishes. In R. J. Wootton & C. Smith (Eds.), Gametogenesis (pp. 45-80). Chichester, England: John Wiley & Sons, Ltd.

Wootton, R. J. & Smith, C. (2014a). Reproductive Biology of Teleost Fishes. In R. J. Wootton & C. Smith (Eds.). Mating systems and sexual selection (pp 201-249). Chichester, England: John Wiley & Sons, Ltd. https://doi.org/10.1002/9781118891360.ch8.

Wootton, R. J. & Smith, C. (2014b). Reproductive Biology of Teleost Fishes. In R. J. Wootton & C. Smith (Eds.), Endocrinology of reproduction (pp. 81-126). Chichester, England: John Wiley & Sons, Ltd.

Xin, N., Liu, T., Zhao, H., Wang, Z., Liu, J., Zhang, Q. & Qi, J. (2014). The impact of exogenous DNA on the structure of sperm of olive flounder (Paralichthys olivaceus). Anim. Reprod. Sci., 149, 305-310. https://doi.org/10.1016/j.anireprosci.2014.06.029.

Yasui, G. S., Arias-Rodríguez, L., Fujimoto, T. & Arai, K. (2009). A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species. Anim. Reprod. Sci., 116(3), 335-345. https://doi.org/10.1016/j.anireprosci.2009.02.021.

Zhang, G., Gurtu, V., Kain, S. R. & Yan, G. (1997). Early detection of apoptosis using a fluorescent conjugate of Annexin V. BioTechniques, 23(3), 525-531.

Zohar, Y. & Mylonas, C. C. (2001). Endocrine manipulations of spawning in cultured fish: from hormones to genes. Aquaculture, 197(1-4), 99-136. https://doi.org/10.1016/S0044-8486(01)00584-1.

Published

2018-05-03

How to Cite

López-Hernández, J. C., Osorio-Pérez, A., Jiménez-Félix, S. A., Páramo-Delgadillo, S., Márquez-Couturier, G., Yasui, G. S., & Arias-Rodríguez, L. (2018). Review article: Fish sperm quality and assessment methods. Revista Ciencias Marinas Y Costeras, 10(1), 67-96. https://doi.org/10.15359/revmar10-1.5

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Scientific articles

How to Cite

López-Hernández, J. C., Osorio-Pérez, A., Jiménez-Félix, S. A., Páramo-Delgadillo, S., Márquez-Couturier, G., Yasui, G. S., & Arias-Rodríguez, L. (2018). Review article: Fish sperm quality and assessment methods. Revista Ciencias Marinas Y Costeras, 10(1), 67-96. https://doi.org/10.15359/revmar10-1.5

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