Feminization of juveniles of White Robalo <i> Centropomus undecimalis</ i> (Bloch 1792) using 17β-Estradiol

  • Juan Manuel Vidal-López Universidad Juárez Autónoma de Tabasco, Mexico
  • Carlos Alfonso Álvarez-González Universidad Juárez Autónoma de Tabasco, Mexico
  • Wilfrido Miguel Contreras-Sánchez Universidad Juárez Autónoma de Tabasco, Mexico
  • Reynaldo Patiño Cooperative Fish and Wildlife Research Units, United States
  • Arlette Amalia Hernández-Franyutti Universidad Juárez Autónoma de Tabasco, Mexico
  • Ulises Hernández-Vidal Universidad Juárez Autónoma de Tabasco, Mexico
  • Rafael Martínez-García Universidad Juárez Autónoma de Tabasco, Mexico
Keywords: Feminization, 17β-estradiol, common snook


Common snook Centropomus undecimalis is a protandric fish with a high commercial importance and aquacultural potential in Mexico and the United States. Several studies indicate that females have higher growth capacity than males. For this reason, the objective of this research was to evaluate the effect of a 17β-estradiol (E2) diet supplementation on sex proportion for this species. In this sense, an experimental study was conducted where fish were fed for different time periods (7, 14, 21, 28, 35, and 42 days) with food impregnated with 50 mg of E2/Kg, and one control diet without the presence of the steroid. After feeding times, fish were raised for additional 204 days with the control diet to evaluate sex proportion, growth and survival. Our results showed that fish fed with E2 for 21 days or more had the highest female sex proportion (93-100%), while the control group showed the highest male proportion (100%). The highest growth (weight and total length) was detected in fish fed with E2 for 21 days (193.11 ± 1.83 mm and 28.56 ± 0.63 g) compared with the rest of the treatments. Survival did not show statistical differences between treatments (92-98%). We conclude that high percentage of C. undecimalis females can be obtained when fish are fed for 21 days or more with artificial food supplemented with E2.


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Author Biographies

Juan Manuel Vidal-López, Universidad Juárez Autónoma de Tabasco

Laboratorio de Acuicultura Tropical, División Académica de Ciencias Biológicas

Carlos Alfonso Álvarez-González, Universidad Juárez Autónoma de Tabasco

Laboratorio de Acuicultura Tropical, División Académica de Ciencias Biológicas

Wilfrido Miguel Contreras-Sánchez, Universidad Juárez Autónoma de Tabasco

Laboratorio de Acuicultura Tropical, División Académica de Ciencias Biológicas

Arlette Amalia Hernández-Franyutti, Universidad Juárez Autónoma de Tabasco

Laboratorio de Acuicultura Tropical, División Académica de Ciencias Biológicas

Ulises Hernández-Vidal, Universidad Juárez Autónoma de Tabasco

Laboratorio de Acuicultura Tropical, División Académica de Ciencias Biológicas

Rafael Martínez-García, Universidad Juárez Autónoma de Tabasco

Laboratorio de Acuicultura Tropical, División Académica de Ciencias Biológicas


Aguilar, M., Coutiño, B. B. & Salinas, R. P. (1996). Manual General de Técnicas Histológicas e Histoquímicas. UNAM. México, D. F.: Las Prensas de Ciencias, Facultad de Ciencias.

Álvarez-Lajonchére, L. S. (2004). Cultivo de robalos: Potencialidades e resultados. Panorama Acuícola, 14(85), 15-21.

Caballero, V. (1996). Biología reproductiva del robalo Blanco (Centropomus undecimalis) en la zona suroeste del estado de Campeche. CRIP Cd. del Carmen. Informe Técnico del Instituto Nacional de la Pesca (inédito). Campeche, México: NAPESCA. Cd. del Carmen.

Chang, C. F. & Lee, M. F. (1992, junio). Involvement of estradiol β 17 on the processes of natural and control sex reversal in protandrous black porgy (Acanthopagrus schlegeli). Ponencia presentada. Second International Symposium on Fish Endocrinology. St. Malo, France.

Chapman, P., Cross, F., Fish, W. & Jones, K. (1982). Study 1: Artificial culture of snook. Final report for sportfish introduction projects. Florida, EE.UU.: Game and Fresh Water, Fish Commission.

Colombo, G. & Grandi, G. (1990). Gonad sex differentiation of Anguilla anguilla by sex steroids. Int. Rev. Hydrobiol., 76, 763-773.

Conley, A. J. & Walters, K. W. (1999). Aromatization. En E. Knobil & J. D. Neill (Eds.), Encyclopedia of Reproduction. Vol. 1 (pp. 280-291). New York, EE.UU.: Academic Press.

Contreras-Sánchez, W. M., Márquez-Couturier, G., Feist, G., Hernández-Franyutti, A., Schreck, C. B. & Giannico, G. (2003). Diversification of Aquacultural practices by incorporation of native species and implementation of alternative sex inversion techniques. Twenty-first Annual technical reports. Corvallis, Oregón, EE.UU.: Aquaculture CRSP.

Contreras-Sánchez, W. M., Hernández-Vidal, U., Hernández-Franyutti, A. A., Álvarez-González, C. A., Márquez-Couturier, G., Paramo-Delgadillo, S., Vidal-López, J. M., Morales-Lara, G., De la Cruz-Rodríguez, M., Castro-Vasconcelos, C. C., Barabata-De la Cruz, J. L., Patiño, R., Pope, K. & Huber, C. (2007). Development of aquaculture techniques for indigenous species of Southern México, Centropomus undecimalis: Sex determination and differentiation and effects of temperature. Seedstock Development & Availability 4 (12SDA4). In K. Kosciuch (Ed.), Aquaculture Collaborative Research Support Program: Sustainable Aquaculture for a Secure Future (pp 212-228). Oregon, EE.UU.: Aquaculture CRSP.

Devlin, R. H. & Nagahama, Y. (2002). Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture, 208(3-4), 191-364.

Flynn, S. R. & Benfey, T. J. (2007). Effects of dietary estradiol-17β in juvenile shortnose sturgeon (Acipenser brevirostrum), Lesueur. Aquaculture, 270, 405-412.

Goudie, C. A., Redner, B. D., Simco, B. A. & Davis, K. B. (1983). Feminization of channel catfish by oral administration of steroid sex hormones. Trans. Am. Fish. Soc., 112, 670-672.

Green, B. W., Veverica, K. L. & Fitzpatrick, M. S. (1997). Fry and fingerling production. In H. S. Egna & C. E. Boyd (Eds.), Dynamics of Pond Aquaculture (pp. 215-243). New York, EE.UU.: CRC Press.

Grier, H. J. & Taylor, R. G. (1998). Testicular maturation and regression in the common snook. J. Fish Biol., 53, 521-542.

Grier, H. J. (2000). Ovarian germinal epithelium and folliculogenesis in the common snook Centropomus undecimalis (Teleostei: Centropomidae). J. Morphol., 234, 265-281.

Happe, A. & Zohar, Y. (1988) Self-fertilization in the protandrous hermaphrodite Sparus aurata: Development of technology. In Y. Zohar & B. Brenton (Eds.), Reproduction in Fish - Basic and Applied Aspects in Endocrinology and Genetics (pp. 177-180). Italy, Paris: INRA Press.

Hendry, C. I., Martin-Robichaud, D. J. & Benfey, T. J. (2003). Hormonal sex reversal of Atlantic halibut (Hippoglossus hippoglossus L.) Aquaculture, 219, 769-781.

Huber, C. G. (2007). Habitat preferences of juvenile common snook in the Lower Rio Grande, Texas. Tesis de maestría no publicada, Texas Tech University, Texas, EE.UU.

Hunter, G. A. & Donaldson, E. M. (1983). Hormonal sex control and its application to fish culture. En W. S. Hoar, D. J. Randall & E. M. Donaldson (Eds.), Fish Physiology, Reproduction, Behavior and Fertility Control (pp. 223-303). New York, EE.UU.: Academic Press.

Islinger, M., Wilimsky, D., Völkl, A. & Braunbeck, T. (2003). Effects of 17α- Ethynilestradiol on the expression of three estrogen-responsive genes and cellular ultrastructure of liver and testes in male zebrafish. Aquatic Tox., 62, 85-103.

Jeyasuria, P. & Place, A. R. (1998). Embryonic brain-gonadal axis in temperature-dependent sex determination of reptiles: a role for P450 aromatase (CYP19). J. Exp. Zool., 281, 428-449.

Johnstone, R., Simpson, T. H. & Youngson, A. F. (1978). Sex reversal in salmonid culture. Aquaculture, 13(2), 115-134.

Marshall, A. R. (1958). A survey of the snook fishery of Florida, with studies of the biology of the principal species, Centropomus undecimalis Bloch. Florida. State Bd. Conser. Tech. Ser., 22, 5-37.

Nakamura, M. (1984). Effects of estradiol-17β on gonadal sex differentiation in two species of salmonids, the masu salmon, Oncorhynchus masou, and the chum salmon, O. keta. Aquaculture, 43(1-3), 83-90.

Pandian, T. J. & Sheela, S. G. (1995). Hormonal induction of sex reversal in fish. Aquaculture. 138, 1-22.

Perera-García, M. A., Mendoza-Carranza, M., Connteras-Sánchez, W. M., Huerta-Ortiz, M. & Pérez Sánchez, E. (2011). Reproductive biology of common snook Centropomus undecimalis (Perciformes: Centropomidae) in two tropical habitats. Rev. Biol. Trop., 59(2), 669-681.

Peters, K. M., Matheson, R. E. Jr. & Taylor, R. G. (1998). Reproduction and early life history of common snook, Centropomus undecimalis (Bloch) in Florida. Bull. Mar. Sci., 62(2), 509-529.

Phelps, R. P., Lovshin, L. L. & Green, B. W. (1996). Sex Reversal of Tilapia: 17α-Methyltestosterone Dose Rate by Environment and Efficacy of Bull Testes. Progress Report, Honduras Special Study 1. Oregon, EE.UU.: Pond Dynamics/Aquaculture Collaborative Research Support Program.

Piferrer, F. (2001). Endocrine sex control strategies for the feminization of teleost fish. Aquaculture, 197(1-4), 229-281.

Rivas, L. R. (1986). Systematic review of the perciform fishes of the genus Centropomus. Copeia, 3, 579-611.

Sánchez, Z. A., Rosas, V. C., Durruty, L. C. V. & Suárez-Bautista, J. (2002). Reproducción en cautiverio de robalo, una necesidad inaplazable en el sureste mexicano. Panorama Acuícola Mag., 6, 24-25.

StatPoint, Inc. (2006). STATGRAPHICS (Data analysis software system), Version 5.1. www.statgraphics.com. Warrenton, EE.UU.: StatPoint, Inc.

Stewart, A. B., Spicer, A. V., Inskeep, E. K. & Dailey, R.A. (2001). Steroid hormone enrichment of Artemia nauplii. Aquaculture, 202(1-2), 177-181.

Strüssmann, C. A., Takashima, F. & Toda, K. (1996). Sex differentiation and hormonal feminization in pejerrey Odontesthes bonariensis. Aquaculture, 139(1-2), 31-45.

Taylor, R. G., Whittington, J. A., Grier, H. J. & Crabtree, R. E. (2000). Age, growth, maturation and protandric sex reversal in common snook, Centropomus undecimalis, from the east and west coasts of Florida. Fish Bull., 98, 612-624.

Tringali, M. D. & Bert, T. M. (1996). The genetic stock structure in common snook (Centropomus undecimalis). Can. J. Fish. Aquatic Sci., 53(5), 974-984.

Tucker, J. W. Jr. (1987). Snook and tarpon snook culture and preliminary evaluation for commercial farming. Prog. Fish. Cult., 49, 49-57.

Tucker, J. W. Jr. (2003). Snook culture. World Aquac. Soc. Mag., 34(4), 42-46.

Vázquez, G. A. (2008). Evaluación del efecto feminizante de la administración del esteroide 17-β estradiol en juveniles del pejelagarto (Atractosteus tropicus). Tesis de Licenciatura no publicada. División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, México.

Vidal-López, J. M., Álvarez-González, C. A., Contreras-Sánchez, W. M. & Hernández-Vidal, U. (2009). Masculinización del cíclido nativo Tenhuayaca, Petenia splendida (Günther, 1862), usando nauplios de Artemia como vehículo del esteroide 17-α metiltestosterona. Hidrobiológica, 19(3), 211-216.

Yamamoto, T. O. (1969). Sex differentiation. En W. S. Hoard & D. J. Randall (Eds.), Fish Physiology (pp. 117-175). New York, EE.UU.: Academic Press.

Zarza, E. A., Berruecos, J. M., Vázquez, C. & Álvarez, P. (2006). Cultivo experimental de robalo Centropomus undecimalis (Bloch, 1792) y Chucumite Centropomus parallelus (Poey, 1860) (Perciformes: Centropomidae) en agua dulce en un estanque de concreto en Alvarado, Veracruz, México. Vet. Méx., 37(3), 327-333.

How to Cite
Vidal-López, J., Álvarez-González, C., Contreras-Sánchez, W., Patiño, R., Hernández-Franyutti, A., Hernández-Vidal, U., & Martínez-García, R. (2012). Feminization of juveniles of White Robalo <i> Centropomus undecimalis</ i&gt; (Bloch 1792) using 17β-Estradiol. Journal of Marine and Coastal Sciences, 4, 83-93. https://doi.org/10.15359/revmar.4.5
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