Effect of two cytokinins and a growth inhibitor on the in vitro tuberization of two genotypes of Solanum tuberosum L. cvs. Atlantic and Alpha
This investigation was carried out to evaluate the effect of two cytokinins: 6-benzilaminopurine (BAP) (6.5 mg l-1) and kinetin (K) (2.5 mg l-1), as well as the growth inhibitor abscisic acid (ABA) (1.0 mg l-1) on the in vitro tuberization capacity of two potato varieties: Atlantic and Alpha. The basal culture medium MS (1962) was used as a control. The responses were different between varieties. In cv. Atlantic, the analysis of the number (NM), weight (WM) and diameter (DM) of microtubers indicated that the addition of growth regulators did not affect induction and development of microtubers. However, when BAP was used, a non-significant increment of 41 % was observed in the number of the microtubers compared to the control treatment, from 2.6 to 4.4. The addition of cytokinins and ABA to the medium did not have a significant impact on the development of microtubers. In cv. Alpha the cytokinins used without ABA increased the number of microtubers, which were larger and heavier than those of the control treatment. In this variety, ABA significantly reduced the values of the NM, WM and DM variables. The exogenous action of cytokinins in the culture medium is likely to have caused an endogenous hormonal imbalance in the Atlantic and Alpha genotypes which interfered with their innate microtuberization ability, a result that was even more evident for cv. Alpha, which showed the need to continue optimizing protocols of genotype-specific systems in potato tissue culture to increase yield and seed quality.
Aksenova, N. P., Konstantinova, T. N., Golyanovskaya, S. A., Sergeeva, L. I., & Romanov, G. A. (2012). Hormonal regulation of tuber formation in potato plants. Russian Journal of Plant Physiology, 59(4), 451–466. https://doi.org/10.1134/S1021443712040024
Aksenova, N. P., Konstantinova, T. N., Lozhnikova, V. N., Golyanovskaya, S. A., Gukasyan, I. A., Gatz, C., & Romanov, G. A. (2005). Photoperiodic and Hormonal Control of Tuberization in Potato Plants Transformed with the PHYB Gene from Arabidopsis. Russian Journal of Plant Physiology, 52(5), 623–628. https://doi.org/10.1007/s11183-005-0092-8
Aksenova, N. P., Konstantinova, T. N., Lozhnikova, V. N., Golyanovskaya, S. A., & Sergeeva, L. I. (2009). Interaction between day length and phytohormones in the control of potato tuberization in the in vitro culture. Russian Journal of Plant Physiology, 56(4), 454–461. https://doi.org/10.1134/S1021443709040037
Al-safadi, B., Ayyoubi, Z., & Jawdat, D. (2000). The effect of gamma irradiation on potato microtuber production in vitro. Plant Cell, Tissue and Organ Culture, 61(3), 183–187. https://doi.org/10.1023/A:1006477224536
Alexopoulos, A. A., Akoumianakis, K. A., & Passam, H. C. (2006). Effect of plant growth regulators on the tuberisation and physiological age of potato (Solanum tuberosum L.) tubers grown from true potato seed. Canadian Journal of Plant Science, 86(4), 1217–1225. https://doi.org/10.4141/P05-227
Brenner, W. G., & Schmülling, T. (2015). Summarizing and exploring data of a decade of cytokinin-related transcriptomics. Frontiers in Plant Science, 6 (January), 1–13. https://doi.org/10.3389/fpls.2015.00029
Chapman, E. J., & Estelle, M. (2009). Cytokinin and auxin intersection in root meristems. Genome Biology, 10(2), 210. https://doi.org/10.1186/gb-2009-10-2-210
Coleman, W. K., & Coleman, S. E. (2000). Modification of potato microtuber dormancy during induction and growth in vitro or ex vitro. American Journal of Potato Research, 77(2), 103–110. https://doi.org/10.1007/BF02853737
Coleman, W. K., Donnelly, D. J., & Coleman, S. E. (2001). Potato microtubers as research tools: A review. American Journal of Potato Research, 78(1), 47–55. https://doi.org/10.1007/BF02874824
Dobránszki, J., Magyar-Tábori, K., & Hudák, I. (2008). In vitro Tuberization in Hormone-Free Systems on Solidified Medium and Dormancy of Potato Microtubers. Fruit, Vegetable and Cereal Science and Biotechnology, 2(1), 82–94.
Donnelly, D. J., Coleman, W. K., & Coleman, S. E. (2003). Potato microtuber production and performance: A review. American Journal of Potato Research, 80(2), 103–115. https://doi.org/10.1007/BF02870209
Ewing, E. E., Simko, I., Omer, E. A., & Davies, P. J. (2004). Polygene mapping as a tool to study the physiology of potato tuberization and dormancy. American Journal of Potato Research, 81(4), 281–289. https://doi.org/10.1007/BF02871770
Finkelstein, R. (2013). Abscisic acid synthesis and response. The Arabidopsis Book, e0166. https://doi.org/10.1199/tab.0166
Gopal, J. (2001). In vitro and in vivo genetic parameters and character associations in potato. Euphytica, 118(2), 145–151. https://doi.org/10.1023/A:1004062900701
Gopal, J., Chamail, A., & Sarkar, D. (2002). Slow-growth in vitro conservation of potato germplasm at normal propagation temperature. Potato Research, 45(2–4), 203–213. https://doi.org/10.1007/BF02736115
Gopal, J., Chamail, A., & Sarkar, D. (2004). In vitro production of microtubers for conservation of potato germplasm: effect of genotype, abscisic acid, and sucrose. In vitro Cellular & Developmental Biology. Plant., 40(5), 485–490. https://doi.org/10.1079/IVP2004540
Gopal, J., Minocha, J. L., & Dhaliwal, H. S. (1998). Microtuberization in potato (Solanum tuberosum L.). Plant Cell Reports, 17(10), 794–798. https://doi.org/10.1007/s002990050485
Jiménez, E., Pérez, N., de Feria, M., Barbón, R., Capote, A., Chávez, M., … Pérez, J. C. (1999). Improved production of potato microtubers using a temporary immersion system. Plant Cell, Tissue and Organ Culture, 59(1), 19–23. https://doi.org/10.1023/A:1006312029055
Kawakami, J., Iwama, K., Jitsuyama, Y., & Zheng, X. (2004). Effect of cultivar maturity period on the growth and yield of potato plants grown from microtubers and conventional seed tubers. American Journal of Potato Research, 81(5), 327–333. https://doi.org/10.1007/BF02870178
Kefi, S., Pavlista, A. D., Meagher, M. M., & Read, P. E. (2000). Short communication invertase activity as affected by cytokinin-like compounds during potato tuberization in vitro. American Journal of Potato Research, 77(1), 57–61. https://doi.org/10.1007/BF02853662
Lê, C. L. (1999). In vitro microtuberization: an evaluation of culture conditions for the production of virus-free seed potatoes. Potato Research, 42(3–4), 489–498. https://doi.org/10.1007/BF02358165
Li, H. Z., Zhou, W. J., Zhang, Z. J., Gu, H. H., Takeuchi, Y., & Yoneyama, K. (2005). Effect of γ-radiation on development, yield and quality of microtubers in vitro in Solanum tuberosum L. Biologia Plantarum, 49(4), 625–628. https://doi.org/10.1007/s10535-005-0062-1
López-Delgado, H. A., Sánchez-Rojo, S., Mora-Herrera, M. E., & Martínez-Gutierrez, R. (2012). Micro-Tuberization as a Long Term Effect of Hydrogen Peroxide on Potato Plants. American Journal of Potato Research, 89(3), 240–244. https://doi.org/10.1007/s12230-011-9219-y
Macháčková, I., Konstantinova, T. N., Sergeeva, L. I., Lozhnikova, V. N., Golyanovskaya, S. A., Dudko, N. D., … Aksenova, N. P. (1998). Photoperiodic control of growth, development and phytohormone balance in Solanum tuberosum. Physiologia Plantarum, 102(2), 272–278. https://doi.org/10.1034/j.1399-3054.1998.1020215.x
Motallebi-Azar, A., Kazemiani, S., & Yarmohamadi, F. (2013). Effect of sugar/osmotica levels on in vitro microtuberization of potato (Solanum tuberosum L.). Russian Agricultural Sciences, 39(2), 112–116. https://doi.org/10.3103/S1068367413020146
Murashige, T., & Skoog, F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Park, S. W., Jeon, J. H., Kim, H. S., Hong, S. J., Aswath, C., & Joung, H. (2009). The effect of size and quality of potato microtubers on quality of seed potatoes in the cultivar “Superior.” Scientia Horticulturae, 120(1), 127–129. https://doi.org/10.1016/j.scienta.2008.09.004
Raghavendra, A. S., Gonugunta, V. K., Christmann, A., & Grill, E. (2010). ABA perception and signalling. Trends in Plant Science, 15(7), 395–401. https://doi.org/10.1016/j.tplants.2010.04.006
Romanov, G. A., Aksenova, N. P., Konstantinova, T. N., Golyanovskaya, S. A., Kossmann, J., & Willmitzer, L. (2000). Effect of indole-3-acetic acid and kinetin on tuberisation parameters of different cultivars and transgenic lines of potato in vitro. Plant Growth Regulation, 32(2–3), 245–251. https://doi.org/10.1023/A:1010771510526
Sarkar, D., Pandey, S. K., & Sharma, S. (2006). Cytokinins antagonize the jasmonates action on the regulation of potato (Solanum tuberosum) tuber formation in vitro. Plant Cell, Tissue and Organ Culture, 87(3), 285–295. https://doi.org/10.1007/s11240-006-9166-3
Scofield, S., Dewitte, W., Nieuwland, J., & Murray, J. A. H. (2013). The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity. Plant Journal, 75(1), 53–66. https://doi.org/10.1111/tpj.12198
Shan, J., Song, W., Zhou, J., Wang, X., Xie, C., Gao, X., … Liu, J. (2013). Transcriptome analysis reveals novel genes potentially involved in photoperiodic tuberization in potato. Genomics, 102(4), 388–396. https://doi.org/10.1016/j.ygeno.2013.07.001
Sharma, A. K., Venkatasalam, E. P., & Singh, R. K. (2011). Micro-tuber production behaviour of some commercially important potato (Solanum tuberosum) cultivars. Indian Journal of Agricultural Sciences, 81(11), 1008–1013.
Sharma, S., Chanemougasoundharam, A., Sarkar, D., & Pandey, S. K. (2004). Carboxylic acids affect induction, development and quality of potato (Solanum tuberosum L.) microtubers grown in vitro from single-node explants. Plant Growth Regulation, 44(3), 219–229. https://doi.org/10.1007/s10725-004-5827-6
Sharma, S., Venkatasalam, E. P., Patial, R., Latawa, J., & Singh, S. (2011). Influence of gelling agents and nodes on the growth of potato microplant. Potato Journal, 38(1), 41–46.
Villafranca, M. J., Veramendi, J., Sota, V., & Mingo-Castel, A. M. (1998). Effect of physiological age of mother tuber and number of subcultures on in vitro tuberisation of potato (Solanum tuberosum L.). Plant Cell Reports, 17(10), 787–790. https://doi.org/10.1007/s002990050483
Xu, X., van Lammeren, A., Vermeer, E., & Vreugdenhil, D. (1998). The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiology, 117(2), 575–584. https://doi.org/10.1104/pp.117.2.575
Yoshida, T., Mogami, J., & Yamaguchi-Shinozaki, K. (2015). Omics approaches toward defining the comprehensive abscisic acid signaling network in plants. Plant and Cell Physiology, 56(6), 1043–1052. https://doi.org/10.1093/pcp/pcv060
Yu, J. W., Choi, J.-S., Upadhyaya, C. P., Kwon, S. O., Gururani, M. A., Nookaraju, A., … Park, S. W. (2012). Dynamic proteomic profile of potato tuber during its in vitro development. Plant Science, 195, 1–9. https://doi.org/10.1016/j.plantsci.2012.06.007
Zhang, Z. J., Li, H. Z., Zhou, W. J., Takeuchi, Y., & Yoneyama, K. (2006). Effect of 5-aminolevulinic acid on development and salt tolerance of potato (Solanum tuberosum L.) microtubers in vitro. Plant Growth Regulation, 49(1), 27–34. https://doi.org/10.1007/s10725-006-0011-9
Zhang, Z., Mao, B., Li, H., Zhou, W., Takeuchi, Y., & Yoneyama, K. (2005). Effect of salinity on physiological characteristics, yield and quality of microtubers in vitro in potato. Acta Physiologiae Plantarum, 27(4), 481–489. https://doi.org/10.1007/s11738-005-0053-z
Zhang, Z., Zhou, W., & Li, H. (2005). The role of GA, IAA and BAP in the regulation of in vitro shoot growth and microtuberization in potato. Acta Physiologiae Plantarum, 27(3), 363–369. https://doi.org/10.1007/s11738-005-0013-7
Zürcher, E., & Müller, B. (2016). Cytokinin synthesis, signaling, and function—advances and new insights. In International review of cell and molecular biology (Vol. 324, pp. 1-38). Academic Press. https://doi.org/10.1016/bs.ircmb.2016.01.001
Authors who publish with this journal agree to the following terms:
1. Authors guarantee the journal the right to be the first publication of the work as licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
2. Authors can set separate additional agreements for non-exclusive distribution of the version of the work published in the journal (eg, place it in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
3. The authors have declared to hold all permissions to use the resources they provided in the paper (images, tables, among others) and assume full responsibility for damages to third parties.
4. The opinions expressed in the paper are the exclusive responsibility of the authors and do not necessarily represent the opinion of the editors or the Universidad Nacional.
Uniciencia Journal and all its productions are under Creative Commons Atribución-NoComercial-SinDerivadas 4.0 Unported.
There is neither fee for access nor Article Processing Charge (APC)
Comentarios (ver términos de uso)
Most read articles by the same author(s)
- José Bernal Azofeifa-Bolaños, Conceptual Evolution and Importance of Andragogy towards the Scope Optimization of University Academic Rural Development Programs and Projects , Revista Electrónica Educare: Vol 21, No 1 (2017): Educare Electronic Journal (January-April)