Effect of pineapple (Ananas Comosus) culture by- products on the enzymatic deproteinization of shrimp biowastes
AbstractThe present work aims to a preliminary study of the deproteinization capacity of proteolytic extracts from pineapple biowastes in different concentrations as potential green process to treat by-products of the shrimp fishery in order to obtain chitin and chitosan. The proteolytic extract isolated from pineapple biowastes was obtained from pineapple stems using a sulfuric acid buffer solution. The proteolytic extract from pineapple was characterized according to its protein content, total sugars and enzymatic activity against a commercial bromelin standard and a negative control. Results indicate that applying a crude pineapple proteolytic extract succeeded in to eliminate up to 97% of the proteins present in a shrimp biowaste sample, being more active than any of its dilutions, in a 24 hours study. Our results suggest that pineapple biowastes can be consider as a potential green biosource to promote shrimp deproteinization, providing a biotechnological activity to the traditional chemical process involving sodium hydroxide. This novel methodology will provide new applications for pineapple biowastes and reduce costs and contamination during chitin and chitosan production.
Authors who publish with this journal agree to the following terms:
1. Authors retain copyright and guaranteeing the magazine the right to be the first publication of the work as licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this magazine.
2. Authors can set separate additional agreements for non-exclusive distribution of the version of the work published in the journal (eg, place it in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
Uniciencia Journal and all its productions are under Creative Commons Atribución-NoComercial-SinDerivadas 4.0 Unported.
Most read articles by the same author(s)
- Daniel Esquivel Alvarado, Gerardo Rodríguez Rodríguez, Sergio Madrigal Carballo, Evaluation of the chemical composition (Phyllanthosides and Lignans) of the crude extract of the Chilillo tree (Phyllanthus Acuminatus Vahl) from different sites of Costa Rica , Uniciencia: Vol 27 No 1 (2013)