Effect of pineapple (Ananas Comosus) culture by- products on the enzymatic deproteinization of shrimp biowastes

Authors

  • Javier Rodrigo Alpízar Cordero Universidad de Ciencias Médicas, Costa Rica
  • Luis Roberto Villegas Peñaranda Universidad Nacional, Costa Rica
  • Sergio Madrigal Carballo
  • María Sibaja Ballestero Universidad Nacional, Costa Rica

Keywords:

Pineapple, bromelin, shrimp, deproteinization, enzymatic activity

Abstract

The present work aims to a preliminary study of the deproteinization capacity of proteolytic extracts from pineapple biowastes in different concentrations as potential green process to treat by-products of the shrimp fishery in order to obtain chitin and chitosan. The proteolytic extract isolated from pineapple biowastes was obtained from pineapple stems using a sulfuric acid buffer solution. The proteolytic extract from pineapple was characterized according to its protein content, total sugars and enzymatic activity against a commercial bromelin standard and a negative control. Results indicate that applying a crude pineapple proteolytic extract succeeded in to eliminate up to 97% of the proteins present in a shrimp biowaste sample, being more active than any of its dilutions, in a 24 hours study. Our results suggest that pineapple biowastes can be consider as a potential green biosource to promote shrimp deproteinization, providing a biotechnological activity to the traditional chemical process involving sodium hydroxide. This novel methodology will provide new applications for pineapple biowastes and reduce costs and contamination during chitin and chitosan production.  

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Published

2013-01-01

How to Cite

Effect of pineapple (Ananas Comosus) culture by- products on the enzymatic deproteinization of shrimp biowastes. (2013). Uniciencia, 27(1), 156-170. https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/4948

Issue

Section

Original scientific papers (evaluated by academic peers)

How to Cite

Effect of pineapple (Ananas Comosus) culture by- products on the enzymatic deproteinization of shrimp biowastes. (2013). Uniciencia, 27(1), 156-170. https://www.revistas.una.ac.cr/index.php/uniciencia/article/view/4948

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