Design of a spectrofluorometric method for the determination of ethanol in air
A simple and rapid method for the determination of ethanol in air was designed, using a chilled glass spiral for sampling and a flow injection system coupled to a column containing alcohol oxidase, immobilized on glass beads and a fluorescence detection system for analysis. We evaluated the excitation wavelength (310 nm) and the emission wavelength (410 nm), the flow of the mobile phase (1,05 mL min-1), the length of the reactor (1,00 m), the length (7,00 cm) and the diameter (3,0 mm) of glass column containing alcohol oxidase and the concentration of sodium hydroxide solution (0,15 M). The analytical method was linear from 1,00 μmol L-1 to 5,00 μmol L-1 ethanol. We obtained a correlation coefficient of 0,995, a repeatability of 0,8 %, a limit of detection and quantification of 0,350 μmol L-1 and 0,670 μmol L-1, respectively. The methodology was evaluated in field sampled and the ethanol concentrations in air ranged from 0,356 ± 0,001 mg/m3 and 1200 ±2 mg/m3. These concentrations did not exceed the maximum allowable limit of 1884 mg/m3, according to OSHA.
Authors who publish with this journal agree to the following terms:
1. Authors retain copyright and guaranteeing the magazine the right to be the first publication of the work as licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this magazine.
2. Authors can set separate additional agreements for non-exclusive distribution of the version of the work published in the journal (eg, place it in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
Uniciencia Journal and all its productions are under Creative Commons Atribución-NoComercial-SinDerivadas 4.0 Unported.
Most read articles by the same author(s)
- Tatiana Vásquez Morera, Rosa Alfaro Solís, José Pablo Sibaja Brenes, Germain Esquivel Hernández, Juan Valdés González, Chemical composition of rain and fog water collected in the Monteverde biological reserve , Uniciencia: Vol 26 No 1-2 (2012)