Producción y excreción de glicolato en chlorella marina (ING)
AbstractThe marine Green alga Chlorella marina (CCAP 211/27) excreted glycollate when incubated under high photon irradiance, high pH and low CO2. The glycollate pathway inhibitors α-hydroxy- 2- pyridine – methanesulfonate (HPMS) and isonicotinyl hydrazide (INH) increased glycollate excretion rates by inhibiting glycollate metabolism. Neither compound affect ribulose biphosphate (RuBP) carboxylase/oxygenase activity, indicating that the inhibitors do not influence glycollate metabolism. Neither compound affected ribulose biphosphate (RuBP) carboxylase/oxygenase activity, indicating that the inhibitors do not influence glycollate formation rates but that glycollate excretion in their presence may be taken as an index of the rate of glycollate production. Previous growth under air or air plus 5% CO2 did not influence rates of glycollate production. The excretion of glycollate obtained with C. marina during photosynthesis under normal conditions of growth, is a demonstration that under those glycollate production and metabolism occur in healthy cells.
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