Efecto de la edad al trasplante y de la concentración de azúcar in vitro sobre la adaptación y el crecimiento de vitroplantas de papa (Solanum tuberosum L.) vars. atlantic y 1625 frito lay en invernadero. (ING)

Abstract

The initial adaptation and posterior in vivo growth and development of plants propagated in vitro is one of the most important steps in the micropropagation process of a plant species. Thus, in this work the main aim was to determine the effect of different ages at transplantation (15, 20 and 25 days), and the effect of pre-conditioning in vitro with three levels of commercial sugar (15, 30 and 45 g L^-1), on the development and growth of potato microplants. In the first case, the MS (1962) culture media (Gibco Laboratories) was used, at a concentration of 17 g L^-1 and supplemented with vitamins and aminoacids of the same media. In the second case, the Hyponex media was employed using 2.25 g L^-1 and supplemented with vitamins and aminoacids of the MS culture media. Results indicated that it is possible to transplant potato plantlets of both varieties at any of the three ages, without affecting tuber yield, although apparent photosynthesis rate was improved by age. Regarding the influence of different levels of sugar, results indicated that plantlets of cv. Atlantic gained more root dry weight and more fresh and dry weight of minitubers with 15 g L^-1 of sugar, whereas plants of cv. 1625 responded better to 30 g L^-1. The number of minitubers was not changing by the sugar concentration, but the MS (1962) culture media was better than Hyponex in this case, the aparent photosynthesis rate showed a slight but consistent gain with increasing concentrations of sugar.