ACE and ACTN3 polymorphisms analysis on agility test in national players in team sports of Costa Rica. Pilot study




team sports, ACE gene, ACTN3 gene, genetics


Objective:  To analyze the differences in the polymorphisms of the ACE and ACTN3 genes on agility test performance in elite players of collective sports from National teams of Costa Rica.

Methods: a sample of 33 male team sports players (futsal, rugby, volleyball, and handball). All subjects were tested with the Illinois Agility Test. Two days of measurements were made; on the first day, cell samples were obtained and on the second day, the agility test was applied. The Chi-square test (x2) was used to determine the differences between the frequencies of the polymorphisms of the ACE and ACTN3 genes and the type of sport.

Results: The highest distribution of polymorphisms of the ECA gene of players from national teams of collective sports was found in the ACE ID (X2 = 6.87, p = .334), and in ACTN3 the RX (X2 = 6.33, p =. 388). Furthermore, no significant relationship was found between the Illinois test performance and the polymorphisms of the ECA gene (F = 2,150, p = .134).

Conclusions: The ACE and ACTN3 genes polymorphisms were not statistically related to the type of team sport. Agility is not associated with the type of polymorphism, which indicates that regardless of the gene, this physical quality can be trained and generate good results in the general population.


Ahmetov, I., Egorova, E., Gabdrakhmanova, L., & Fedotovskaya, O. (2016). Genes and Athletic Performance: An Update. Medicine and Sport Science, 61, 41-54.

Aidar, M., & Line, S. (2007). A simple and cost-effective protocol for DNA isolation from buccal epithelial cells. Brazilian Dental Journal, 18(2), 148-152.

Alfred, T., Ben-Shlomo, Y., Cooper, R., Hardy, R., Cooper, C., Deary, I., Gunnell, D., Harris, S., Kumari, M., Martin, R., Moran, C., Pitsiladis, Y., Ring, S., Sayer, A., Smith, G., Starr, J., Kuh, D., & Day, I. (2011). ACTN3 genotype, athletic status, and life course physical capability: Meta-analysis of the published literature and findings from nine studies. Human Mutation, 32(9), 1008-1018.

Arroyo, W. (2021). Genética y fútbol: Asociación de los polimorfismos genéticos ACTN3 y ACE-I/D en jugadores de fútbol: Revisión literaria. Retos, 39.

Barbosa, D., Mendoza, E., Cruz, I., Magalhães, B., Kappes, L., Cruz, E., Santos, M., & Silami, E. (2018). Evidence for a Role of ACTN3 R577X Polymorphism in Football Player’s Career Progression. International Journal of Sports Medicine, 39(14), 1088-1093.

Caló, M., & Vona, G. (2008). Gene polymorphisms and elite athletic performance. Journal of Anthropological Sciences = Rivista Di Antropologia : JASS, 86, 113-131.

Cięszczyk, P., Leońska-Duniec, A., Maciejewska-Skrendo, A., Sawczuk, M., Leźnicka, K., Contrò, V., Trybek, G., & Lulińska-Kuklik, E. (2016). Variation in the ACE gene in elite Polish football players. Human Movement, 17(4), 237-241.

Dawes, J. (2019). Developing Agility and Quickness (2nd Edition). Human Kinetics.

Dionísio, T., Thiengo, C., Brozoski, D., Dionísio, E., Talamoni, G., Silva, R., Garlet, G., Santos, C., & Amaral, S. (2017). The influence of genetic polymorphisms on performance and cardiac and hemodynamic parameters among Brazilian soccer players. Applied Physiology, Nutrition, and Metabolism, 42(6), 596-604.

Egorova, E., Borisova, A., Mustafina, L., Arkhipova, A., Gabbasov, R., Druzhevskaya, A., Astratenkova, I., & Ahmetov, I. (2014). The polygenic profile of Russian football players. Journal of Sports Sciences, 32(13), 1286-1293.

Eynon, N., Alves, A., Yamin, C., Sagiv, M., Duarte, J., Oliveira, J., Ayalon, M., Goldhammer, E., Sagiv, M., & Meckel, Y. (2009). Is there an ACE ID - ACTN3 R577X polymorphisms interaction that influences sprint performance? International Journal of Sports Medicine, 30(12), 888-891.

Eynon, N., Banting, L., Ruiz, J., Cieszczyk, P., Dyatlov, D., Maciejewska-Karlowska, A., Sawczuk, M., Pushkarev, V., Kulikov, L., Pushkarev, E., Femia, P., Stepto, N., Bishop, D., & Lucia, A. (2013). ACTN3 R577X polymorphism and team-sport performance: A study involving three European cohorts. Journal of Science and Medicine in Sport, 17(1), 102-106.

Eynon, N., Ruiz, J., Femia, P., Pushkarev, V. P., Cieszczyk, P., Maciejewska-Karlowska, A., Sawczuk, M., Dyatlov, D., Lekontsev, E., Kulikov, L., Birk, R., Bishop, D., & Lucia, A. (2012). The ACTN3 R577X polymorphism across three groups of elite male European athletes. PloS One, 7(8), e43132.

Eynon, N., Ruiz, J. R., Oliveira, J., Duarte, J. A., Birk, R., & Lucia, A. (2011). Genes and elite athletes: A roadmap for future research. The Journal of Physiology, 589(13), 3063-3070.

Ferreira, A., Caniuqueo, A., Hernández, C., da Silva, S., Izquierdo, M., Silva, H., Ramirez-Campillo, R., & Fernandes, J. (2015). Polimorfismo del Gen ACTN3 y ECA en Seleccionados de Gimnasia de Brasil y Japón. International Journal of Morphology, 33(1), 262-266.

Galeandro, V., Notarnicola, A., Bianco, A., Tafuri, S., Russo, L., Pesce, V., Moretti, B., & Petruzzella, V. (2017). ACTN3/ACE genotypes and mitochondrial genome in professional soccer players’ performance. Journal of Biological Regulators and Homeostatic Agents, 31(1), 207-213.

Gineviciene, V., Jakaitiene, A., Aksenov, M., Aksenova, A., Druzhevskaya, A., Astratenkova, I., Egorova, E., Gabdrakhmanova, L., Tubelis, L., Kucinskas, V., & Utkus, A. (2016). Association analysis of ACE, ACTN3 and PPARGC1A gene polymorphisms in two cohorts of European strength and power athletes. Biology of Sport, 33(3), 199-206.

Gineviciene, V., Jakaitiene, A., Tubelis, L., & Kucinskas, V. (2014). Variation in the ACE, PPARGC1A and PPARA genes in Lithuanian football players. European Journal of Sport Science, 14(1), S289-295.

Gunel, T., Gumusoglu, E., Hosseini, M., Yilmazyildirim, E., Dolekcap, I., & Aydinli, K. (2014). Effect of angiotensin I-converting enzyme and α-actinin-3 gene polymorphisms on sport performance. Molecular Medicine Reports, 9(4), 1422-1426.

Heffernan, S., Kilduff, L., Erskine, R., Day, S., McPhee, J., McMahon, G., Stebbings, G., Neale, J., Lockey, S., Ribbans, W., Cook, C., Vance, B., Raleigh, S., Roberts, C., Bennett, M., Wang, G., Collins, M., Pitsiladis, Y., & Williams, A. (2016). Association of ACTN3 R577X but not ACE I/D gene variants with elite rugby union player status and playing position. Physiological Genomics, 48(3), 196-201.

Jeremic, D., Zivanovic, I., Vulovic, M., Stevanovic, J., Radovanovic, D., Varjacic, V., & Djordjevic, D. (2019). ACE/ACTN3 genetic polymorphisms and athletic performance of female soccer players`. Revista Brasileira de Medicina Do Esporte, 25(1), 35-39.

Koku, F., Karamızrak, S., Çiftçi, A. S., Taşlıdere, H., Durmaz, B., & Çoğulu, Ö. (2019). The relationship between ACTN3 R577X gene polymorphism and physical performance in amateur soccer players and sedentary individuals. Biology of Sport, 36(1), 9-16.

Ma, F., Yang, Y., Li, X., Zhou, F., Gao, C., Li, M., & Gao, L. (2013). The association of sport performance with ACE and ACTN3 genetic polymorphisms: A systematic review and meta-analysis. PloS One, 8(1), e54685.

Macarthur, D., & North, K. (2005). Genes and human elite athletic performance. Human Genetics, 116(5), 331-339.

Massidda, M., Corrias, L., Scorcu, M., Vona, G., & Calò, M. (2012). ACTN-3 and ACE genotypes in elite male Italian athletes. Anthropological Review, 75(1), 51-59.

Moraes, V. N., Trapé, A. A., Ferezin, L. P., Gonçalves, T. C. P., Monteiro, C. P., & Bueno, C. R. (2018). Association of ACE ID and ACTN3 C>T genetic polymorphisms with response to a multicomponent training program in physical performance in women from 50 to 70 years. Science & Sports, 33(5), 282-290.

Morales, M., Sentchilo, V., Bertelli, C., Komljenovic, A., Kryuchkova-Mostacci, N., Bourdilloud, A., Linke, B., Goesmann, A., Harshman, K., Segers, F., Delapierre, F., Fiorucci, D., Seppey, M., Trofimenco, E., Berra, P., Taher, A. E., Loiseau, C., Roggero, D., Sulfiotti, M., Etienne, A., Ruiz, G., Pillard, L., Escoriza, A., Moritz, R., Schneider, C., Alfonso, E., Jeddou, F., Selmoni, O., Resch, G., Greub, G., Emery, O., Dubey, M., Pillonel, T., Robinson-Rechavi, ,M. & van der Meer, J. R. (2016). The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand. PLOS ONE, 11(11), e0165850.

Orozco-Diaz, A., Moncada-Jiménez, J., & Leal, A. (2019). Allelic and genotypic frequencies of Actinin 3 gene (ACTN3) in the Costa Rican population and its association with physical fitness in college students. Biology of Exercise, 15(1). 1-12.

Orysiak, J., Mazur-Różycka, J., Busko, K., Gajewski, J., Szczepanska, B., & Malczewska-Lenczowska, J. (2018). Individual and Combined Influence of ACE and ACTN3 Genes on Muscle Phenotypes in Polish Athletes. Journal of Strength and Conditioning Research, 32(10), 2776-2782.

Papadimitriou, I. D., Lucía, A., Pitsiladis, Y., Pushkarev, V., Dyatlov, D., Orekhov, E., Artioli, G., Guilherme, J., Lancha, A., Ginevičienė, V., Cieszczyk, P., Maciejewska-Karlowska, A., Sawczuk, M., Muniesa, C., Kouvatsi, A., Massidda, M., Calò, C., Garton, F., Houweling, P., Wang, G., Austin, K., Druzhevskaya, A., Astratenkova, I., Ahmetov, I., & Eynon, N. (2016). ACTN3 R577X and ACE I/D gene variants influence performance in elite sprinters: A multi-cohort study. BMC Genomics, 17, 285.

Santiago, C., González-Freire, M., Serratosa, L., Morate, F., Meyer, T., Gómez-Gallego, F., & Lucía, A. (2008). ACTN3 genotype in professional soccer players. British Journal of Sports Medicine, 42(1), 71-73.

Sheppard, J., & Young, W. (2006). Agility literature review: Classifications, training and testing. Journal of Sports Sciences, 24(9), 919-932.

Stewart, P., Turner, A., & Miller, S. (2014). Reliability, factorial validity, and interrelationships of five commonly used change of direction speed tests. Scandinavian Journal of Medicine & Science in Sports, 24(3), 500-506.

Ulucan, K., Sercan, C., & Biyikli, T. (2015). Distribution of Angiotensin-1 Converting Enzyme Insertion/Deletion and α-Actinin-3 Codon 577 Polymorphisms in Turkish Male Soccer Players. Genetics & Epigenetics, 7, 1-4.

Yang, N., MacArthur, D., Gulbin, J., Hahn, A., Beggs, A., Easteal, S., & North, K. (2003). ACTN3 genotype is associated with human elite athletic performance. American Journal of Human Genetics, 73(3), 627-631.



How to Cite

Carvajal-Espinoza, R., Trejos-Montoya, J., Herrera-González, E., Alemán-Ramírez, C., Márquez-Barquero, M., Cervantes-Sanabria, J., Loria-Calderón, T., Miranda-Benavides, G., & Gutiérrez-Vargas, R. (2023). ACE and ACTN3 polymorphisms analysis on agility test in national players in team sports of Costa Rica. Pilot study. MHSalud: Revista En Ciencias Del Movimiento Humano Y Salud, 20(1), 1-10.

How to Cite

Carvajal-Espinoza, R., Trejos-Montoya, J., Herrera-González, E., Alemán-Ramírez, C., Márquez-Barquero, M., Cervantes-Sanabria, J., Loria-Calderón, T., Miranda-Benavides, G., & Gutiérrez-Vargas, R. (2023). ACE and ACTN3 polymorphisms analysis on agility test in national players in team sports of Costa Rica. Pilot study. MHSalud: Revista En Ciencias Del Movimiento Humano Y Salud, 20(1), 1-10.

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